Production,partial purification and bioassay of toxic metabolites of three plant pathogenic species ofColletotrichum in Nigeria

Colletotrichum lindemuthianum, C. truncatum andC. graminicola, produced metabolites in culture which induced necrotic lesions on susceptible hosts. The highest production was obtained from Richard's medium under shake incubation. The toxic metabolites of these pathogens fluoresce at 254 nm and 366 nm under ultraviolet light. The crude metabolites of theseColletotrichum species inhibited seed germination at the concentration of 100 µg/ml while the potency of the metabolites decreases with increase in dilution to 0.1 µg/ml. Similarly, the metabolites also inhibited growth of seedlings of hosts to the pathogen at 100 µg/ml and the potency again reduced with increasing dilution to 0.10 µg/ml.     

Regulation of photosynthetic carbon assimilation in leaves of C3–C4 intermediate species ofMoricandia andFlaveria

The relationship between the gas-exchange characteristics, the contents of photosynthetic intermediates and the quantum yield of photosystem II was examined at different intercellular partial pressures of CO₂ (p _i) in attached leaves of Moricandia arvensis L. (D.C.) and Flaveria floridana J.R. Johnson (both C₃–C₄ intermediate plants) and, for comparison, in F. pringlei Gandoger (a C₃ plant) and in F. bidentis (a C₄ plant). Both C₃–C₄ intermediate species had pools of phosphoenolpyruvate, pyruvate, alanine and aspartate intermediate to those of the C₃ and C₄ species examined. Moricandia arvensis had large pools of glycine at low p _i, consistent with the operation of a glycine shuttle from mesophyll to bundle-sheath cells. It also had a high pool of triose-phosphate at ambient partial pressures of CO₂, indicating that a glycerate-3-phosphate/triose-phosphate shuttle could operate in this species. This was not the case in F. floridana. A decline in the ribulose-1,5-bisphosphate and triose-phosphate pool in M. arvensis, and a rise in the pools of glycerate-3-phosphate and pyruvate in F. floridana, at low p _i, show different patterns of metabolic regulation in M. arvensis and F. floridana at low p _i in comparison to C₃ and C₄ plants.Abbreviations Frul,6bisP fructose-1,6-bisphosphate - PEP phosphoenolpyruvate PGA-glycerate-3-phosphate - p _i intercelular CO₂ pressure - PPFD photosynthetic photon flux density; - RuBP ribulose-1,5-bisphosphate - triose-P triose phosphates This work was done while R.C.L. was a Visiting Fellow at the Australian National University, and was sponsored by the Royal Society. We are grateful to Kathy Britt for assistance with the analysis of amino acids.     

谷胱甘肽的解毒作用与毒性代谢物

谷胱甘肽是细胞内重要的含SH基的非蛋白分子,它对氧自由基、有机氢过氧化物以及亲电子剂的灭活起着极重要的作用.但近年来的研究表明,一些连位二卤烷烃、卤烯烃、含酮结构的化合物以及一些异氰酸酯、异硫氰酸酯、醛、α,β－不饱和醛等与谷胱甘肽轭合后可导致毒性代谢物的形成.     

塔里木盆地胀果甘草内生放线菌多样性及抗菌活性分析

【目的】发掘具有开发前景的放线菌资源,对分离自新疆胀果甘草的内生放线菌的多样性、抗菌活性和次级代谢产物合成相关基因进行研究。【方法】采用5种培养基和3种前处理方法,从胀果甘草中分离获得80株放线菌。基于菌株形态学特征,对36株代表菌株进行抗菌活性检测,通过特异性引物扩增方法,检测了PKS I、PKS II、NPRS和卤化酶基因,探究其合成天然产物的潜在能力。结合筛选结果,选取其中20株代表菌,经16S r RNA基因测序,对其进行系统发育分析。【结果】培养基E2和E3结合热处理的分离效果较好;86.1%的代表菌株对供试的细菌、病原真菌表现出了不同程度的抗菌活性,PKS I、PKS II、NRPS基因和卤化酶基因阳性检出率分别为16.7%、72.2%、25.0%和11.1%。具有活性功能的代表菌株经16S r RNA基因测序分析,分别属于链霉菌属(Streptomyces)、小单胞菌属(Micromonospora)、红球菌属(Rhodococcus)和游动放线菌属(Actinoplanes)4个属,其中链霉菌属(Streptomyces)为优势菌属,占60%以上。【结论】胀果甘草是我国传统的药用植物,其植株内部蕴藏着丰富的放线菌资源,并在次生代谢产物合成方面拥有巨大潜力,具有进一步开发的价值。     

A sucrose transporter‐interacting protein disulphide isomerase affects redox homeostasis and links sucrose partitioning with abiotic stress tolerance

Sucrose accumulation in leaves in response to various abiotic stresses suggests a specific role of this disaccharide for stress tolerance and adaptation. The high‐affinity transporter StSUT1 undergoes substrate‐induced endocytosis presenting the question as to whether altered sucrose accumulation in leaves in response to stresses is also related to enhanced endocytosis or altered activity of the sucrose transporter. StSUT1 is known to interact with several stress‐inducible proteins; here we investigated whether one of the interacting candidates, StPDI1, affects its subcellular localization in response to stress: StPDI1 expression is induced by ER‐stress and salt. Both proteins, StSUT1 and StPDI1, were found in the detergent resistant membrane (DRM) fraction, and this might affect internalization. Knockdown of StPDI1 expression severely affects abiotic stress tolerance of transgenic potato plants. Analysis of these plants does not reveal modified subcellular localization or endocytosis of StSUT1, but rather a disturbed redox homeostasis, reduced detoxification of reactive oxygen species and effects on primary metabolism. Parallel observations with other StSUT1‐interacting proteins are discussed. The redox status in leaves seems to be linked to the sugar status in response to various stress stimuli and to play a role in stress tolerance.     

慢性疲劳综合征中学生运动前后尿液差异代谢物的比较

目的：探讨慢性疲劳综合征（CFS）中学生运动前后尿液的差异代谢物及其代谢通路特征,阐述慢性疲劳综合征的代谢机制。方法：依据美国疾病预防控制中心关于CFS的筛选标准,选取8名17~19岁男性CFS中学生作为受试对象,同时选择来自同一学校的8名同龄、同性别健康中学生作为对照组。受试者进行一次改良的哈佛台阶运动（上下台阶30次/min,持续3 min）,采集运动前后的尿液,以液相-质谱联用（LC-MS）法检测其差异代谢物;采用多维统计法对所检测到的代谢物进行主成分分析（PAC）和正交偏最小二乘判别分析（OPLS-DA）;通过MetPA数据库分析与差异代谢物相关的代谢通路。结果：与对照组相比较,运动前CFS组筛选出肌酸、吲哚乙醛、植物鞘氨醇和焦谷氨酸4个差异代谢物,其含量均显著下降（P<0.05或P<0.01）;运动后CFS组检测出11个差异代谢物,依次是壬二酸、甲基腺苷、乙酰肉碱、癸酸、皮质酮、肌酸、左炔诺孕酮、泛酸、焦谷氨酸、黄嘌呤核苷和黄尿酸,其中除甲基腺苷和肌酸比对照组显著升高（P<0.05）外,其他9个差异代谢物均较对照组显著降低（P<0.05或P<0.01）。将上述15个差异代谢物分别输入MetPA数据库进行代谢通路权重得分分析,结果显示,运动前CFS组只检测出精氨酸-脯氨酸代谢通路紊乱,标记代谢物为肌酸;而运动后检测出精氨酸-脯氨酸代谢、泛酸与辅酶A生物合成、类固醇激素生物合成3条代谢通路存在障碍,标记代谢物依次是：肌酸、泛酸和皮质酮。结论：运动干预前,CFS中学生的精氨酸-脯氨酸代谢通路紊乱被检出;施加运动后,又检测出其类固醇激素生物合成代谢通路与泛酸和辅酶A代谢通路也存在代谢紊乱情况。     

构树叶性状分化的生态功能初步研究

叶性状分化在自然界中较为普遍,不同的叶性状特征与植物对资源获得及利用效率密切关联,反映了植物适应特定环境所形成的生存对策。叶性状分化的生态功能一直以来备受生态学家和进化生物学家的广泛关注。自然界构树(Broussonetia papyifera)在个体发育过程中出现全缘叶和裂缺叶的分化,但其生态功能尚不清楚,推测两者的叶型分化是构树对虫害规避的结果。为了探讨构树叶性状分化对应的可能生态功能,该研究采用野外监测和室内分析的方法,对构树全缘叶和裂缺叶的虫害发生率、叶面积、与抗虫有关的酚类物质(总酚、缩合单宁、黄酮)含量进行了比较。结果表明:(1)相对于裂缺叶,全缘叶虫害发生率显著增加,全缘叶虫害发生率是裂缺叶的两倍。(2)自然条件下,全缘叶叶面积显著高于裂缺叶,增加了约44个百分点。(3)自然条件下,裂缺叶中总酚、缩合单宁、黄酮含量均显著高于全缘叶,分别提高了6.0%、4.2%和16.2%。(4)除黄酮外,虫害处理下裂缺叶中总酚、缩合单宁含量显著高于全缘叶,均提高了约5.0%。(5)人为移除部分叶片,裂缺叶中总酚、缩合单宁、黄酮含量均显著高于全缘叶,分别提高8.0%、1.6%和25.4%。这说明构树全缘叶和裂缺叶中酚类物质含量对外来损伤响应不一致,裂缺叶虫害发生率较全缘叶低可能由于两种类型叶片中酚类物质含量存在差异所引起。